BiomedGrid

  Automatic Generation of Drug Concentration Gradient by a Modified Ink-jet Printer and its Application in Drug Screening Abstract Manual preparation of drug concentration gradient is labor-intensive and limits the efficiency of a drug screening to some extent, especially at large scale. In this study, we reported an automatic method to generate drug concentration gradient in 24-well plate. We loaded drug solution in a modified hot ink-jet printer, designed printing pattern in the WORD software, customized the concentration gradient in the WORD software by RGB coding, and finally printed the drug concentration gradient in the 24-well plate. A drug concentration gradient can be automatically generated within 5s in arbitrary pattern. Based on this flexible technology, we developed an ink-jet printer-based drug screening assay. Concentration gradient of 5-fluorouracil, as well as Mcf-7 cells, were printed in the 24-well plate, and the inhibition rate of Mcf-7 cells was measured by MTT...

  Acidic Pharmaceuticals Simultaneous Removal from Water Sample by Magnetic Ionic Liquid Nanoadsorbent 1-Butyl-3-Aminopropyl Imidazolium Tetrachloroferrate Functionalized Graphene Oxide GO-sBapim) FeCl 4 Abstract This paper evaluated the removal efficiency of twelve acidic pharmaceuticals by microextraction with 1-butyl-3-aminopropyl imidazolium tetrachloroferrate functionalized graphene oxide GO-(Bapim)FeCl 4  nanocomposite. The acidic pharmaceuticals studied were salicylic acid (SYL), clofibric acid (CFA), ibuprofen (IBP), acetaminophen (AAP), gemfibrozil (GFB), fenoprofen (FNP), naproxen (NPX), triclosan (TCS), ketoprofen (KTP), diclofenac (DOF), fenofibrate (FFB), and indomethacin (IDM), which are commonly used as analgesic/anti-inflammatory agents or lipid regulators. The proposed procedure complies with the principles of the green chemistry, since it uses low volumes of easily synthesized MILsbased magnetic extracting phases avoiding the use of toxic solvents. The analyt...