Biomedical Science and Research Journals | Automatic Generation of Drug Concentration Gradient by a Modified Ink-jet Printer and its Application in Drug Screening
![Image](https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEieUfE-wsmW-HjFVbVjBh6O2rnWQluPKCafI7AnYfj8TbkRqSQqOfayEKVd4EuGWjiv_mXSa1gv7YsRO12GOpmjym20w_N-4flvjT-97jx9UiVETTsiWS0i5b6aP3Amc3jlop-3TudhLTI/w577-h305/image.png)
Automatic Generation of Drug Concentration Gradient by a Modified Ink-jet Printer and its Application in Drug Screening Abstract Manual preparation of drug concentration gradient is labor-intensive and limits the efficiency of a drug screening to some extent, especially at large scale. In this study, we reported an automatic method to generate drug concentration gradient in 24-well plate. We loaded drug solution in a modified hot ink-jet printer, designed printing pattern in the WORD software, customized the concentration gradient in the WORD software by RGB coding, and finally printed the drug concentration gradient in the 24-well plate. A drug concentration gradient can be automatically generated within 5s in arbitrary pattern. Based on this flexible technology, we developed an ink-jet printer-based drug screening assay. Concentration gradient of 5-fluorouracil, as well as Mcf-7 cells, were printed in the 24-well plate, and the inhibition rate of Mcf-7 cells was measured by MTT met