Biomedical Science and Research Journals | Assessment of 3 Tac-I/Beta, an Antimicrobial Peptide Source, in the Control of Pseudomonas Plant Pathogens

Diseases caused by Pseudomonas syringae and other species of Pseudomonas cause severe losses in different fruit crops worldwide. Antimicrobial peptides (AMPs) are small and consist of a variety of proteins that act on the lipid bilayer of the bacterial membrane that is produced by multicellular organisms. The product, 3 Tac-I/Beta, is a biopesticide that bases its action on three Trichoderma strains (T. viride strain T-26, T. harzianum strain T-22, and T. longibrachiatum strain T-397), its total concentration is >25 x 103 c.f.u. g-1, which is authorized in Chile to control different fungal diseases in fruit and vegetable crops. However, the reduction of bacterial stone canker disease has suggested a possible AMPs mode of action associated with 3 Tac-I/Beta. In this study we used Transmission Electronic Microscopy (TEM) to assess the possible AMP activity of this product on the bacterial membrane of Pseudomonas syringae pv. syringae (Pss). The effect of two filtered solutions of 3 Tac-I/Beta (commercial and concentrated) on the population of this bacterium was assessed by using in vitro tests.
Control on this bacterial plant pathogen in cherry plants cv. Bing treated with 3 Tac-I/Beta, copper hydroxide, and copper oxide was assessed in a pot experiment. This TEM study showed a marked damage on the membrane of P. syringae pv. syringae, which affected the bacillus shape of the bacterium by changing the continuity of its membrane. In vitro tests on a homogeneous lawn of Pss showed the formation of inhibition halos around discs treated with filtered 3 Tac-I/Beta across the surface of agar King B-medium plates. Reduction in the bacterial population after 18 hours of bacterial growth in King B medium amendment with a solution of commercial and concentrated filtered of 3 Tac-I/Beta were observed. Bacterial growth, expressed as the optical density of cell culture, was reduced by around 12% when 3 Tac-I/Beta was used, while a concentrated AMP solution of filtered 3 Tac-I/Beta showed a reduction of around 34.8% after 18 hours of co-culture. The effect on bacterial growth was maintained for 30 hours for both filtered solutions of the product. Preventive spraying of 3 Tac-I/Beta on cherry plants cv. ‘Bing’.

Comments

Post a Comment

Popular posts from this blog

Biomedical Science and Research Journals | Advanced Applications of Biomaterials Based on Alginic Acid

Advanced Applications of Biomaterials Based on Alginic Acid Abstract Alginic acid, also known as algin or alginate, is a natural carbohydrate, which is derived from marine brown algae, as well as some microorganisms. It can be used in various applications. In particular, alginate has shown great potential in the areas of wound healing, drug delivery, in vitro cell culture, and tissue engineering, allowing the categorization of it as a promising biomaterial, or a basic component of other biomaterials. The unique characteristics of alginic acid, such as the biocompatibility, the mild required gelation conditions, the low toxicity, the relative low cost and the simple modifications, allow the development of alginate hydrogels, and alginate derivatives with enhanced properties. The aim of this review, is to present an overview of the properties of alginate, shedding light on the current and potential applications and suggesting new perspectives for future studies. Introduction Innovative b
Read more

Biomedical Science and Research Journals | The Need of Early Detection of Positive COVID-19 Patients in the Community by Viral Tests (e.g. RT-PCR Tests) and Antibody Tests (Serological Tests) to Stop the Spread

Image
  The Need of Early Detection of Positive COVID-19 Patients in the Community by Viral Tests (e.g. RT-PCR Tests) and Antibody Tests (Serological Tests) to Stop the Spread Abstract Background and Objective:  The Aim of this article is to tell the Importance of early testing for COVID-19 novel corona virus of the suspected communities. The earlier to find out the Positive results of the COVID-19 novel corona virus more robust preventions against COVID-19 could be implemented. Methods:  The Author of this article has randomly selected 15 Articles from PUBMED Central and others sources with search word COVID-19 Test Kits. After reviewing the research articles and looking at their results it clearly shows the Importance of use of COVID-19 Test Kits. The earliest the use of COVID-19 Test Kits it prevents the whole community from getting infection from infected people. The Positive people with COVID-19 Test Kits would be Quarantined and treated in the health facilities or at home depending upo
Read more

Biomedical Science and Research Journals | Substance Identification in Anti-Doping Control-Some Issues

  Substance Identification in Anti-Doping Control-Some Issues Introduction When an athlete is accused of an anti-doping rule violation according to the World Anti-Doping Code (WADC), usually the only evidence available to the ruling body is the presence of a prohibited substance, metabolite or marker in the athlete’s sample, as determined by a laboratory accredited by the World Anti-Doping Agency (WADA). The laboratory enjoys a presumption, which means that if the analysis has been conducted according to WADA’s International Standard for Laboratories and technical documents, it cannot be challenged by the athlete. It speaks for itself that given the enormous consequences for an athlete to be found guilty of an antidoping violation, and the singular importance attributed to the laboratory, the analysis procedure and the underpinning science must be impeccable. Small molecules such as anabolic steroids and their metabolites are usually detected by means of Gas Chromatography (GC) coupled
Read more