BiomedGrid

  Rapid Induction of Orthotopic Hepatocellular Carcinoma in Mice by Fixed Puncture Point Injection Abstract Aim:  Animal model is an indispensable tool in investigating the development, progression and treatment of human cancer including hepatocellular carcinoma (HCC). However, it is still challenging in establishing orthotopic HCC mouse models. Here, we presented a minimally invasive method with high tumor formation rate in mouse liver. Methods:  Hep3B-luc and SNU-449-luc HCC cells mixed with Matrigel were injected into the liver of nude mice from different source at fixed injection site and depth, respectively. The mice were divided into 3 groups. At fifteen days post-injection, the bioluminescence signal of liver tumor was detected and tumors in injected mouse liver were harvested. Cons and pros were then compared between presented and common approaches. Results:  We successfully developed an orthotopic transplantation tumor model in nude mice by directly injectin...

  To Develop an App or Technology Platform for Interprofessional Education & Collaboration for Research Outside Universities & Create Partnerships with Organizations to Create New Learning and Career Opportunities for Dental Hygienists Opinion The oral-systemic link and pathogenesis of inflammation are the key drivers for the body's diseases. Dental hygienists are one of the most underutilized professions in healthcare today. Dental hygienists have a broad base of education with medical and dental knowledge that will be the key conduit to provide preventative healthcare. Dental hygienists must take continuing education courses in several medical and health topics to maintain their licenses every two to three years depending on the state they live in. Some of the educational courses consists of medical emergencies, aseptic techniques, pharmacology, anesthesia, cardiopulmonary respiration, diabetes, oral pathology, and many others. In the future, the American Dental Hygiene A...

  Health Related Quality of Life Perceived Among Caregivers and Breast Cancer Patients in Pakistan: Time to Ponder Abstract Introduction:  Breast cancer has been considered as an emerging non-communicable threat in Pakistan. Limited access to health care facilities, a smaller number of qualified oncologists, lack of technical equipment for diagnosis are the major factors effecting adequate control and prevention of breast cancer in Pakistan. Objective:  The present study was designed to assess health related quality of life among patients of breast cancer in Pakistan. Methodology:  A descriptive cross sectional study design was used to assess health related quality of life among 382 breast cancer patients and 71 care givers using EORTC QLQ C 30. Data was collected and statistically analyzed. Results:  Out of 453 respondents 84.3%, (n=382) were patients while 15.7%, (n=71) were care givers. Out of all the patients, 11.3% (n=43) had stage 1 of breast cancer and 70...

  droTAG: Adapting dTAG Toolkit to Drosophila Melanogaster Opinion Targeted protein degradation (TPD) allows an acute and reversible knockdown of protein of interest (POI) so that the direct effects of protein depletion can be studied and distinguished from secondary effects or adaptive responses [1]. Therefore, protein degradation techniques apply in studying the function of gene products in a short time frame, and rapid effectiveness can be exploited to downregulate POI in a stage-specific manner or when time is a relevant factor, including cell division. In the last decade, several strategies have been developed to obtain an accurate and efficient protein degradation, such as deGradFP, Auxin-inducible Degradation (AID) and degradation TAG (dTAG) which aim to achieve proteolysis of POI exploiting the powerful of degradation signal peptide sequences (tags) to hijack POI to E3 ubiquitin ligases for ubiquitylation and consequentially proteasomal degradation by recruitment of the ubi...